Caught this Rainbow Scarab beetle yesterday and photographed it in the grass with my Z9 and Z 100-400 f4.5-5.6 at 400mm. Just thought I would share as I was surprised how sharp it was. Hard to think I need to drag a macro lens around for occasional shots like this. Your thoughts?
Normally macro is considered 2:1 or more magnified. What this means is a 70 mm wide subject fits edge to edge on your full frame sensor. That combines both closer minimum focus and more magnification. The Nikon Z macro lenses - referred to as MC lenses - get you to 1:1 magnification without any accessories. This means a 35mm wide subject fills the sensor edge to edge without cropping.
Your 100-400 has a magnification ratio of 0.38 times which means 2.63:1 magnification. Filling the frame would mean a subject that is 93mm in length. You can crop the image and increase the resulting size of the image compared to the frame edges, but you are losing detail and it limits your ability to make larger prints. A rainbow scarab beetle is 3/4 of an inch or 22 mm long.
If you made the exact same photo with an MC lens or other macro lens at closest focus distance, you would have a much more magnified image. That's good and bad as it means technique needs to be more precise, AF needs to be very accurate, and you might use focus shift to stack images. And with the same lens, you could back up and get the framing of your original image if that's what you prefer. But you have the option of much higher magnification - and could crop even deeper.
Here is your photo cropped to the approximate dimensions of the same image with a macro lens.
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It depends on what you want to do. A lot of people are perfectly happy with a close up image such as 0.38 -0.75 magnification. That's what is typically used for photos of frogs, butterflies, dragonflies, flowers, etc. One of the big advantages of the 100-400 is the ability to use it as a close up lens for photos with these types of subjects. But if you want to photograph jewelry, stamps, coins, or high magnification of insects, a macro lens is very different.