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Sure, I've used extension tubes to decrease the minimum focusing distance on many of my prime lenses including the 300mm PF it let's you get that much closer to fill the frame with small subjects. You of course lose the ability to focus at long distances when the tube is mounted but that's normal with extension tubes. When I want to shoot macro subjects with my 300mm PF I attach the 36mm Kenko AF extension tube, when I just want to get a bit closer to subjects that aren't truly macro scale I'll use one of the shorter tubes in the Kenko set.
Sure, as posted above you can focus stack manually or using Nikon's Focus Shift Shooting feature in compatible cameras as long as you use extension tubes which support AF functionality which includes Kenko or Vello extension tubes.
